Hild p 12
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Hild p 12
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WebMay 1, 2014 · The primers were labeled P LtetO-hilD UP and P LtetO-hilD DO (Supplementary Table S2). The PCR product was treated with DpnI to remove template traces. ... Proteins were resolved by Tris–Tricine–polyacrylamide gel electrophoresis (PAGE) using 12% gels. Conditions for protein transfer have been described elsewhere . Optimal dilutions of ...
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