Web1 Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an ... WebActive, N-terminal GST-tagged recombinant human Raf-1 residues 306-end, containing the mutations Y340D & Y341D, for use in Kinase Assays. View Price and Availability. ... ATPase/GTPase Activity Assay Kit. sufficient for 200 colorimetric tests. View Price and Availability. Roche. 11162306001. ATP-γ-S =90% (HPLC), solution, pkg of 200 μL (20 …
Glutathione Fluorescent Detection Kit - Thermo Fisher Scientific
WebTo evaluate the ability of the test compounds to compete with CDNB for GST-α-catalyzed or GST-π-catalyzed conjugation to GSH, a GST assay was performed (Figures 3 and 4). The IC 50 values for FBuEA in the presence of GST-α and GST-π were 17.5 μM and 6.0 μM, respectively . FBuEA is more readily catalyzed by GST-π than by GST-α. WebGlutathione-S-transferase (GST) tagged proteins have variety of applications including purification of fusion proteins. The GST pull-down assay is an in vitro method used to determine a physical interaction between a GST tagged probe protein to an unknown or a known target protein. The procedure involves incubation of the GST fusion protein … richard allen charged
INSTRUCTIONS Pierce™ GST Protein Interaction Pull-Down Kit
WebGSTIN VERIFICATION API TO VERIFY GST NUMBERS IN YOUR SOFTWARE OR APPLICATION. We provide easy to use API to retrieve details of any GST number. You … WebDec 19, 2024 · The GST pull-down assay is an intuitive and fast in vitro method for analyzing direct protein–protein interactions and is comprised of a “bait” which is a GST-fused protein expressed in E. … WebThe Active Ras Pull-Down and Detection Kit includes purified GST-Raf1 Ras-binding domain (RBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/washing buffer, anti-Ras antibody, SDS sample buffer, spin columns and collection tubes. The kit was validated using lysates from NIH 3T3 cells, a ... richard allen case