Web1. Day 0: Prepare cells for transfection: seed 63 106 HEK293T/17 cells in 10 mL of complete cell growth medium in 10 cm cell culture plate. Incubate for 16 h at 37Cwith5%CO 2. 2. Day 1: In the ... WebGreiner Bio well tissue culture plate greiner bio one 662160 cellstar Well Tissue Culture Plate Greiner Bio One 662160 Cellstar, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations.
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WebGilson Inc multichannel pipette gilson fa10011 8 channels Multichannel Pipette Gilson Fa10011 8 Channels, supplied by Gilson Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. WebCorning Life Sciences 40um macrolab berkeley n a dulbecco s phosphate buffered salt pbs solution 1x corning 21031cv edta 40um Macrolab Berkeley N A Dulbecco S Phosphate … pabellon universitario vitoria
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WebMar 19, 2024 · To understand the role of the HIV-1 capsid in viral replication, we developed a protocol to biochemically track capsid in the nucleus during infection. To this end, we separated HIV-1-infected cells into nuclear and cytosolic fractions. Fractions were analyzed by western blotting for HIV-1 capsid content as well as for nuclear and cytosolic ... WebCells from GSI and washout conditions were washed with 1 x PBS (Corning, cat# 21031CV) and lysed with 350 μl RLT Plus buffer (Qiagen) supplemented with 10% 2-mercaptoethanol (Sigma, cat# M6250), vortexed briefly, snap-frozen on dry ice, and stored at -80°C. Subsequently, total RNA was isolated using the RNeasy Plus Micro Kit … WebCorning™ Cell Culture Buffers: Dulbecco's Phosphate-Buffered Salt Solution 1X -With low endotoxin content Manufacturer: Corning™ 21031CV : RM70 BD... Jump to Sections of … pabellon y raclette