WebRoche: packaging: pkg of 50 μL (50 nmol; 1mM) Quality Level: 100 : storage temp. −20°C: Analysis Note: ... Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin … WebMay 7, 2024 · 萝卜和甘蓝属间杂种的创建和研究-作物遗传育种专业毕业论文.docx,摘 摘 要 萝h属(Raphanus)中存在赘许多对芸苔属(Brassica)栽培作物有利的性状和基 因,翔缓施缓雄往不育及恢复基鞠、自交不亲和基因耨多种抗逆基因。萝卜(是sativus L.,2n=18,RR)与甘蓝型油菜(最napus L,2n=38,AACC)杂交很难获褥杂莘中及 ...
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WebRoche biotin nick translation mix kit Biotin Nick Translation Mix Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. … WebSep 9, 2015 · All FISH probes were prepared by nick translation labeling using modified nucleotides conjugated with biotin or digoxigenin utilizing biotin nick translation mix (Cat# 11745824910, Roche, USA) for chromosome 4 control probe; digoxigenin nick translation mix (Cat# 11745816910, Roche, USA) for PAICS locus probe, respectively. Probe DNA … how to send same email to several recipients
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WebNick translation (NT) was the first method developed for incorporating radiolabeled nucleotides into DNA (Rigby et al., 1977) (see Fig. 5). Escherichia coli DNA polymerase … WebNov 5, 2013 · The B-probe was labeled through the nick translation procedure using digoxigenin-16-dUTP (DIG-Nick Translation Mix, Roche Applied Science). The PCR mixture consisted of 1 × Taq DNA polymerase buffer (Invitrogen); 2 mM MgCl 2 ; 40 μM dTTP, dGTP and dCTP; 20 μM dATTP; 20 μM biotin-14-dATP; 2 μM DOP primer; and 2 … WebDec 6, 2024 · The probe was labeled with dUTP-biotin (Roche, Basel, Switzerland) by a Nick Translation Kit (Abbott Laboratories, Lake Bluff, IL, USA) using the manufacturer’s protocol. The probe for the (GATA) 8 microsatellite motif was commercially synthesized and terminally labeled with biotin by Macrogen (Seul, South Korea). how to send sat scores to fgcu